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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital role in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants were reported but comprehensive nucleotide sequence data will not be still readily available.
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Blocking peptides are peptides that bind specifically towards the focus on antibody and block antibody binding. These peptide ordinarily incorporates the epitope recognized from the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to guarantee the absolute best outcomes are...
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This guideline outlines further attainable optimization of cellular and immunoassay parameters to be certain the very best results are...
Antibodies bound to the blocking peptide no longer bind into the epitope over the target protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is particular.
Target Information and facts Cyclin E belongs towards the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute for the temporal coordination of get more info every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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